【摘要】 目的:建立HPLC法测定舒胸分散片中人参皂苷Rg1的含量。方法:以舒胸分散片为研究对象,采用Diamonsil C18(200×4.6mm,5μm)色谱柱,流动相:乙腈∶水(0.05%磷酸)=25∶75,流速1.0mL/min,检测波长203nm,柱温为35℃。结果:人参皂苷Rg1在27.52~440.4μg/mL(r=0.9999)范围内呈良好线性关系;平均回收率为97.1%。结论:本测定方法简便、快速、准确,为舒胸分散片质量评价提供可靠方法。更多还原

【Abstract】 Objective:An RP-HPLC method was developed to determine the contents of ginsenoside Rg1 in Shuxiong dispersible tablets.Methods:Diamonsil C18 (200×4.6mm,5μm) column was used,the mobile phase:CH3CN:H2O(0.05% phosphoric acid)=25∶75,at a flow rate of 1.0mL/min.The UV detection wavelength was 203 nm,and the column temperature was 35℃.Results:Th elinear range was 27.52~440.4g/mL (r=0.9999)for ginsenoside Rg1.The average recoveries of ginsenoside Rg1 were 97.1% respectively. Conclusion:The method was found to be simple and accurate for analysis of ginsenoside Rg1 in Shuxiong dispersible tablets,and provided a reliable way for evaluating the quality of it.更多还原