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变形杆菌菌落计数方法的探讨

EXPLORATION ON THE METHOD OF THE COLONY COUNT OF BACILLUS PROTEUS

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【作者】 封会茹董晓根赵伟周翠莲

【Author】 FENG Hui-ru,DONG Xiao-gen,ZHAO Wei,et al.(Center for Disease Control and Prevention of Fengtai,Beijing 100071,China)

【机构】 北京市丰台区疾病预防控制中心北京市丰台区疾病预防控制中心 北京100071北京100071

【摘要】 [目的]探讨一种更精确的变形杆菌菌落计数方法。[方法]依据变形杆菌的特性,采用SS平板培养和试管证实试验对其进行计数,并用传统的营养琼脂斜面底部冷凝水培养法作参照。[结果]SS平板抑制变形杆菌的迁徙性生长,得到单个菌落,有助于准确计数,试管证实试验对变形杆菌进行鉴别确证,排除其他细菌的干扰。[结论]新方法的计数结果在数量级上与传统方法相吻合,而且计数结果更加精确。

【Abstract】 [Objective]To study a new method of counting colony of bacillus proteus.[Methods]SS plate culture and tube test were used to screen the bacillus proteus,the traditional agar assay with condensate water on slant bottom was adopted as the control.[Results]Single colony was obtained from SS plate culture,which facilitated the colony count,and the bacillus proteus was identified by tube test.[Conclusion]It shows that the counting effect of the new method is more precise than that of the traditional.

  • 【文献出处】 现代预防医学 ,Modern Preventive Medicine , 编辑部邮箱 ,2008年01期
  • 【分类号】R378.2
  • 【被引频次】8
  • 【下载频次】311
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