【摘要】 目的研究葛根素对脑缺血/再灌注损伤的保护作用。方法本实验采用大鼠右侧大脑中动脉阻塞(MCAO)2 h,再灌注24 h模型,每组最终保证8只成活大鼠。MCAO前,药物组大鼠分别腹腔注射葛根素25,50,100 mg.kg-1,尼莫地平0.7 mg.kg-1,1次.d-1,连续7 d。操作后,分别用Longa’s法、红四氮唑(TTC)染色法及干燥失重法来评价大鼠的神经功能状态、脑梗塞面积及脑水肿。结果与模型组比较,葛根素50,100 mg.kg-1可明显减少大鼠MCAO后脑梗塞面积、降低脑水肿的程度及改善神经功能症状,具有显着性差异(P<0.05或P<0.01)。结论葛根素对大鼠脑缺血/再灌注损伤具有保护作用。更多还原

【Abstract】 OBJECTIVE Research the protective effects of puerarin on cerebral ischemia-reperfusion injury in rats.METHODSIn this study,cerebral ischemia-reperfusion injury model was made by 2 hours of middle cerebral artery occlusion(MCAO) followed by 24 hours of reperfusion in rats′right cerebral.There were 8 rats alive every group after making model.Rats were pretreated with puerarin 25,50,100 mg·kg-1,nimodipine 0.7 mg·kg-1 once a day for 7 days and then subjected to MCAO.We examined neurological infarction sizes by 2,3,5-triphenyltetrazolium chloride(TTC) staining technique,the cerebral water contents measuring by wet/dry weighing method,the neurological deficit scores grading on a scale of 0–4.The effects of puerarin at the dosage of 25,50 and 100 mg·kg-1 were compared respectively with nimodipine 0.7 mg·kg-1 and model group.RESULTS Puerarin 50 and 100 mg·kg-1 could obviously decrease infarction volumes,neurological deficit scores and cerebral water contents after MACO(P< 0.05 or P<0.01 vs.model group).CONCLUSION Puerarin could protect against the cerebral ischemia-reperfusion injury in rats.更多还原