【摘要】 目的建立高效液相色谱法测定葶苈降血脂胶囊中熊果酸的含量。方法采用Diamonsil ODS色谱柱;流动相为乙腈-甲醇-水(65∶10∶25,每100 mL加乙酸铵0.5 g);检测波长为220 nm。结果熊果酸进样量在1.592~7.960μg内与峰面积积分值呈良好的线性关系(r=0.999 9);平均加样回收率为99.80%(RSD=1.89%,n=6)。结论方法简便、准确,可作为该制剂的质量控制方法。更多还原

【Abstract】 OBJECTIVE To establish an HPLC method for the determination of ursolic acid in Tingli Jiangxuezhi capsules.METHODS The Diamonsil ODS column was used as the stationary phase with the mobile phase of acetonitrile-methanol-water(65∶10∶25,ammonium acetate 0.5 g·100 mL-1).The detection wavelength was 220 nm.RESULTS The linear range of ursolic acid was 1.592~7.960 μg with the correlation coefficient 0.999 9 between the injection quantity and the peak area.The average recovery was 99.80% with the RSD of 1.89%(n=6).CONCLUSION The method is convenient and accurate.It can be used for the quality control of ursolic acid in Tingli Jiangxuezhi capsules.更多还原