【摘要】 目的建立应用大孔吸附树脂纯化齐墩果酸的方法。方法以齐墩果酸的吸附量,齐墩果酸的含量和齐墩果酸的提取率为考察指标,确定大孔吸附树脂提取齐墩果酸的工艺条件。结果AB-8型树脂对女贞子中齐墩果酸有良好的吸附分离性能,可用于提取女贞子中齐墩果酸的工业化生产,其工艺条件为:齐墩果酸最大吸附量为108mg·g-1,吸附流速为2BV·h-1,洗脱剂为95%乙醇,解吸流速为2BV·h-1。洗脱液经浓缩,乙醇溶解,活性炭脱色,重结晶得到结晶性粉末齐墩果酸,熔点为306~308℃,产品纯度大于98%,提取率平均达89.58%。用IR表征了产物结构。结论该工艺简单易行,提取率高,可用于女贞子中齐墩果酸的提取。更多还原

【Abstract】 OBJECTIVE This study aimed to develop a purification method of oleanolic acid with macroreticular absorption resin.METHODS The absorption efficiency,the contents and the extraction yields of oleanolic acid were studied to confirm the optimum purification conditions with macroreticular resin.RESULTS Experiments showed that AB-8 resin had a obvious better absorption and a separation efficiency for oleanolic acid in herbal Ligustrum lucidum and could be used in the industrialization.The optimum extraction conditions were listed as follows:the maximum absorption content of oleanolic acid was 108 mg·g-1,the absorption and desorption flow rate were both controlled at 2 BV·h-1,95% ethanol was used for eluent.After the concentrated solution was further concentrated,dissolved in ethanol,decolorized by activated charcoal and recrystallized,a final product,crystalline powder of oleanolic acid was obtained with melting point for 306℃-308℃,and the purity quotient for the product was >98 %.In these processes,the optimum extraction efficiency of 89.58% was obtained,which have potential industrial application prospect.An IR spectrum was used to characterize the product.CONCLUSION We have developed a simple and convenient purification method of oleanolic acid in herbal Ligustrum lucidum with macroreticular resin with excellent extraction efficiency.更多还原