【摘要】 目的采用原子力显微镜(atomic force microscopy AFM)研究不同处理方法对牙本质表面形貌及粗糙度的影响,为牙本质表面处理方式的临床应用提供理论依据。方法将离体前磨牙牙本质表面分为三组,分别采用磷酸处理,磷酸/次氯酸钠处理,未做任何处理的表面作为空白对照组,在接触模式下(contact mode),采用原子力显微镜观察表面形貌,应用AFM的自带软件测量其表面粗糙度,粗糙度结果经t检验对各组进行统计学分析。结果牙本质表面经过磷酸处理后,牙本质小管变大,粗糙度增加,经过磷酸/次氯酸钠处理后,牙本质表面呈现均一的微观表面,粗糙度显著增加,各组之间均存在统计学差异。结论磷酸处理后能通过牙本质小管的脱矿来增加牙本质表面的粗糙度,而磷酸/次氯酸钠处理后,能在牙本质表面形成均匀的孔隙,极大地增加了表面粗糙度,后者为指导临床中的牙本质表面处理方式提供了新思路。更多还原

【Abstract】 Objective To observe the morphology and roughness of dentinal surface with different treatments and provide clinical application for dentin surface treatments.Methods Extracted premolars were divided into three groups and two groups were treated with phosphoric acid and phosphoric acid/sodium hypochlorite respectively,another was served as control group.Surface morphology was observed by AFM in contact mode and surface roughness was measured by software.All details were analyzed by T-test.Results After treatment with phosphoric acid,dentinal tubule was enlarged and roughness was increased.In phosphoric acid /sodium hypochlorite group,the roughness of dentinal surface was increased significantly.There is statistical difference between each group.Conclusion Phosphoric acid treatment increases dentinal surface roughness through demineralization of dentinal tubule,while phosphoric acid/sodium hypochlorite treatment produces uniform pores on surface of dentin,and increases surface roughness enormously.Our data provide a new study to guide our clinical application for dentin surface treatment from micro point of view.更多还原