【摘要】 学术背景:软组织缺损的修复是整形外科最重要的难题,目前的各种方案都存在一定缺陷,脂肪组织工程技术为解决上述难题提供了一种新的途径。目的:分析脂肪组织工程的研究现状及目前方案的缺陷,对其将来可能的临床应用进行相应展望。检索策略:应用计算机检索Medline数据库1991-01/2007-07相关组织工程化脂肪组织构建的文献,检索词"fat,Tissue engineering",限定文章语言种类为English。同时计算机检索中国期刊全文数据库1991-01/2007-07的相关文献,检索词"脂肪,组织工程",限定文章语言种类为中文。共检索到英文文献241篇,中文文献101篇。阅读标题和摘要进行初筛,纳入标准:与组织工程化脂肪组织构建相关的文献。排除重复研究276篇。文献评价:保留66篇中英文文献进一步分析,最终纳入符合标准的文献30篇。资料综合:构建组织工程化脂肪的要素包括细胞类型、支架和血液供应及微环境。脂肪组织的缺损和不足是常年困惑整形外科医生的问题。通常采用的自体游离组织移植中,移植物的吸收限制了其进一步应用。组织工程技术的发展为解决上述问题提供了可能的途径。目前,鉴于重建美容外科的巨大需求,脂肪组织的组织工程构建已经逐渐成为了一个研究的焦点。结论:组织工程化的脂肪组织真正应用于临床,移植物必须能长年保持正常的形态和功能,组织工程化的脂肪将能为临床彻底解决软组织缺损等难题展示美好的前景。更多还原

【Abstract】 BACKGROUND: The repair of soft tissue defect is disturbing plastic surgeons for a long period of time. Each strategy has its own limitation. Adipe authoritative journals were included. Repetitive articles and Meta analysis articles were excluded. Finally 34 articles werose tissue engineering technique provides a novel resolving approach. OBJECTIVE: To analyze the research status of adipose tissue engineering and their defects, and to prospect the possible application in clinic. RETRIEVAL STRATEGY: A computer-based online search of Medline was undertaken to identify articles about adipose tissue construction published in English from January 1991 to July 2007 with the keywords "fat, tissue engineering". Meanwhile, we searched Chinese Journal Full-text Database for the Chinese articles dated from January 1991 to July 2007 with the same keywords in Chinese. 241 English and 101 Chinese articles were collected. The titles and abstracts were firstly read to select the articles about the construction of tissue-engineered adipose tissue, and 276 repetitive studies were excluded. LITERATURE EVALUATION: Sixty-six Chinese and English articles were selected for further analysis, and finally 30 articles were included. DATA SYNTHESIS: Cell types, scaffold, blood supply and microenvironment are factors for constructing tissue-engineered fat. Adipose tissue defect and insufficiency are two obstacles for the plastic surgeons for a long time. Autologous free fat transplantation is a common therapy, but its resorption limits the further application. Tissue engineering is a possible technique to resolve this problem. At present, the tissue-engineered construction of adipose tissue has become a study focus due to the great demand of plastic surgery. CONCLUSION: Graft remaining normal appearance and function for a long period of time can realize the application of tissue-engineered adipose tissue in clinic. Tissue-engineered adipose tissue is promising to resolve the problems like soft tissue defects in clinic.更多还原