【摘要】 目的探讨苦参碱联合伊马替尼对K562细胞红系分化的影响。方法分别用0.2 g/L苦参碱与0.2μmol/L伊马替尼单独或联合作用K562细胞48 h,观察联苯胺染色阳性细胞数,并利用RT-PCR检测转录因子GFi-1B表达。结果苦参碱可降低伊马替尼单独作用K562细胞的联苯胺染色阳性细胞数以及转录因子GFi-1B的表达。结论苦参碱联合伊马替尼作用K562细胞后对红系分化有一定抑制作用。更多还原

【Abstract】 Objective It is to investigate the effect of matrine in combination with imatinib on erythroid differentiation of K562 cells.Methods The number of positive K562 cells of Benzidine staining was after the cells were respectively exposured to 0.2 g/L matrine,0.2 μmol/L imatinib,and both of them for 48h.Semi-quantitative reverse transcription polymerase chain reaction was performed to detect the expression of GFI-1B.Results Compared with imatinib alone,co-treatment with matrine and imatinib decreased the number of benzidine staining-positive cells and the expression of GFi-1b in K562 cells.Conclusion Matrine in combination with imatinib can inhibit imatinib-induced erythroid differentiation in K562 cells.更多还原