【摘要】 采用正交实验对回流法提取酸枣仁皂苷和黄酮的条件进行了优化,然后从7种不同的大孔吸附树脂中筛选出一种吸附和解吸性能良好的树脂——三菱SP700树脂,又研究了SP700对酸枣仁中皂苷和黄酮2类活性物质的吸附动力学、吸附等温线以及解吸动力学。皂苷和黄酮提取的最佳条件分别是:φ(乙醇)=90%、v(乙醇溶液,mL)∶m(酸枣仁,g)=20∶1、45℃、6.5 h;φ(乙醇)=70%、v(乙醇溶液,mL)∶m(酸枣仁,g)=15∶1、30℃、3.5 h;SP700对皂苷和黄酮的吸附容量分别可达128.5 mg/g和114.0 mg/g;皂苷和黄酮在SP700上的吸附在常温下进行2 h即可达到平衡;皂苷和黄酮在SP700上的吸附最适合的浓度范围分别是8~10 mg/mL和3~5 mg/mL;经过拟合发现,Langmuir模型较Freundlich模型更适合描述2种物质在SP700上的吸附过程,因此2种物质的吸附过程是以单分子层吸附为主;70%的丙酮可以将2种物质从SP700全部解吸下来;同样地,皂苷和黄酮在树脂上的解吸选择在室温下进行,2 h即可达到完全解吸。更多还原

【Abstract】 The extract conditions of saponins(flavonoids) from Suanzaoren by reflux were optimized by an orthogonal design.One resin,Mitsubishi SP700,with excellent performance of adsorption and desorption was selected from seven different kinds of macroporous resin.The adsorption dynamics,adsorption isotherm and desorption dynamics of SP700 were studied.The optimum extract conditions of saponins(flavonoids) from Suanzaoren were:φ(ethanol)=90%、v(ethanol solution,mL)∶m(Suanzaoren,g)=20∶1、45℃、6.5 h(φ(ethanol)=70%、v(ethanol solution,mL)∶m(Suanzaoren,g)=15∶1、30℃、3.5 h).The adsorption capacity of SP700 was 128.5 mg saponins(114.0 mg flavonoids)/g resin.At room temperature,the adsorption of saponins and flavonoids on the resin reached balance only in two hours.The optimum concentration of saponins(flavonoids) for adsorption was 8～10 mg saponins(3～5 mg flavonoids)/mL extract solution.Adsorption isotherm was also determined and Langmuir isothermal adsorption equation was more suitable than Freundlich for describing the adsorption process of saponins and flavonoids on SP700.This proved that the monomolecular layer adsorption happened on the surface of SP700.The saponins and flavonoids adsorbed on SP700 could be desorbed completely by 70% acetone solution.Desorption of saponins and flavonoids from SP700 also reached balance only in two hours at room temperature.更多还原