【摘要】 目的:探讨RhoA在卵巢癌细胞SKOV3体外侵袭和迁移过程中的作用。方法:构建RhoA真核表达载体,以脂质体介导转染卵巢癌细胞SKOV3,用RT-PCR和Westernblot检测SKOV3RhoAmRNA及蛋白表达水平;Boyden小室体外侵袭实验和划痕实验检测细胞侵袭和迁移能力。结果:转染RhoA的实验组与转染空载体的对照组比较,RhoAmRNA的表达丰度(RhoA/GAPDH)为12.01±1.72,明显高于对照组的6.81±1.24;RhoA蛋白的表达水平(RhoA/β-actin)为4.81±0.56,亦明显高于对照组的3.02±0.32,两者差异都有统计学意义(P<0.05)。Boyden小室体外侵袭实验中实验组与对照组平均侵袭百分数分别为(47.60±1.47)%和(22.60±2.40)%(P<0.05),比较划痕后0h与24h的宽度差,实验组和对照组愈合百分比分别为(65±6.4)%和(54±5.5)%(P<0.05);表明转染RhoA后,细胞侵袭和迁移能力明显增强。结论:RhoA增强了卵巢癌细胞SKOV3体外侵袭和迁移能力。更多还原

【Abstract】 Objective:To investigate the effects of RhoA in SKOV3 cell invasion and migration in vitro.Methods:Lipofect-2000 was used for the transfer of RhoA into SKOV3 cells.The expressions of RhoA mRNA and protein in transfected SKOV3 were detected respectively by reverse transcription-polymerase chain reaction (RT-PCR) and Western blot.Boyden chamber in vitro invasion assay and wound-healing assay were used to evaluate the invasive and migratory capability of SKOV3.Results:The transfer of RhoA into SKOV3 resulted in:(1)the increased expression of mRNA and protein of RhoA in transfected SKOV3 obviously (RhoA mRNA/GAPDH 12.01±1.72 vs 6.81±1.24;RhoA protein/β-actin 4.81±0.56 vs 3.02±0.32.P<0.05),(2)the enhanced invasion and migration capacity in vitro.The percentage of invasion and wound closure in RhoA transfected cells were significantly higher compared those of vector transfectd cells[(47.60±1.47)% vs (22.60±2.40)%;(65±6.4)% vs (54±5.5)%;P<0.05].Conclusion:RhoA transfection may increase the invasion and migration capacity of SKOV3 in vitro.更多还原