【摘要】 研究中药有效成分人参皂甙Rg1、肉桂酸和丹参酮IIA组合对人成骨肉瘤MG-63细胞增殖抑制和相关基因表达影响,探索其对肿瘤细胞的生物学效应。以33ìg/ml人参皂甙Rg1、296.32ìg/ml肉桂酸和0.3ìg/ml丹参酮IIA的组合(简称RCT)处理人成骨肉瘤MG-63细胞,以肿瘤细胞分化诱导物HMBA处理MG-63细胞为平行对照,用流式细胞仪、免疫细胞化学检测及光镜观察系统研究RCT组合对MG-63细胞的作用。生长曲线及细胞周期检测显示RCT组合可显著抑制MG-63细胞的增殖,细胞生长抑制率达72.37%,细胞周期阻滞于G0/G1期;免疫细胞化学检测显示RCT组合处理后MG-63细胞的癌基因c-fos、c-myc表达下调,抑癌基因p27、Rb表达上调。RCT组合对MG-63细胞增殖及相关基因表达的影响与分化诱导物六亚甲基双乙酰胺(HMBA)处理组相似。更多还原

【Abstract】 To explore the anticancer mechanisms and biological effects of the effective component of chinese herbs, the human osteosarcoma MG-63 cells were treated with the combination of 33 ìg/ml ginsenoside Rg1, 296.32 ìg/ml cinnamic acid and 0.3 ìg/ml tanshinoneIIA(RCT) and the MG-63 cells treated with hexamethylene bisacetamide(HMBA) were investigated as positive control of induced differentiation.They were subjected to flow cytometry, immuncocytochemical assay and light microscopy.The analysis of cell growth curve and cell cycle revealed that RCT can markedly inhibit cell proliferation and arrest cells in G0/G1 phase.The inhibitory rate of cell growth was 72.37% after 7 days treatment by RCT.The results of immunocytochemical staining indicated that the expression of oncogenes including c-fos and c-myc were down-regulated, whereas the expression of antitumor genes including p27 and Rb were up-regulated.Similar results were obtained from HMBA treated MG-63 cells.更多还原