【摘要】 目的:观察蒲黄总黄酮(Pollen Typhaetotal flavones,PTF)对脂肪细胞糖脂代谢和过氧化物酶体增生物激活受体(peroxisome proliferator-activated receptor,PPAR)家族基因表达的影响,并分析其改善胰岛素抵抗的可能机制。方法:蒲黄总黄酮干预3T3-L1脂肪细胞,实时定量荧光多聚酶联反应检测脂肪细胞分化相关基因PPAR家族,包括PPARα、PPARγ和PPARβ/δ mRNA的表达。结果:蒲黄总黄酮可上调PPARα、PPARγ mRNA的表达,下调PPARβ/δ mRNA的表达,与正常对照组比较,差异有统计学意义(P<0.01)。结论:蒲黄总黄酮可能通过提高3T3-L1脂肪细胞PPARα和PPARγ mRNA的表达改善胰岛素抵抗。更多还原

【Abstract】 Objective:To explore the effects of Pollen Typhae total flavones (PTF) on the mRNA expressions of peroxisome proliferator-activated receptors (PPARs) α, γ, β/δ so as to analyze its possible mechanism in improving the insulin sensitivity of 3T3-L1 adipocytes. Methods: Adipocytes were treated with PTF, and expressions of PPARα、PPARγ、PPARβ/δ mRNAs relating to the adipocyte glucose and lipid metabolism were determined by reverse transcription polymerase chain reaction. Results: PTF obviously up-regulated the expressions of PPARα and PPARγ mRNAs, all showing significant differences as compared with those in the normal control group (P<0.01). Conclusion: With its function as an insulin sensitizer, PTF may enhance the PPARα and PPARγ mRNA expressions in 3T3-L1 adipocytes.更多还原