【摘要】 目的:探讨苦参碱对SPCA/Ⅰ人肺腺癌细胞系的抗增殖作用以及不同浓度苦参碱与化疗药物联合应用时的协同效应。方法:应用甲基噻唑基四唑(methyl thiazolyl tetrazolium,MTT)比色法检测不同浓度苦参碱以及苦参碱与化疗药物联合应用对SPCA/Ⅰ人肺腺癌细胞生长的抑制作用,波长定于570nm,测定光密度(A值)并计算其抑制率。结果:不同浓度的苦参碱在体外对人肺腺癌细胞均有抑制作用,其抑制率与浓度呈正相关。0.1倍血药峰值浓度(peak plasma concentration,PPC)及1倍PPC的苦参碱分别与化疗药物(1倍PPC)联用,体外对人肺腺癌细胞的抑制率均显著高于单用化疗药物。结论:苦参碱在体外对SPCA/Ⅰ人肺腺癌细胞有直接的抑制作用,该药与化疗药物联合应用有协同抗肿瘤效应。更多还原

【Abstract】 Objective:To study the activities of matrine and anti-tumor drugs on SPCA/Ⅰ human lung adenocarcinoma cell line.Methods:Suppression effects of different concentrations of matrine and matrine combined with anti-tumor drugs on lung cancer cells were measured by methyl thiazolyl tetrazolium(MTT)colorimetric assay.Results:Different concentrations of matrine could inhibit the growth of SPCA/Ⅰ human lung adenocarcinoma cells and there was a positive correlation between the inhibition rate and the drug concentration.Different concentrations of matrine combined with anti-tumor drugs had higher growth inhibition rate than anti-tumor drugs alone.Conclusion:Matrine has direct growth suppression effect on SPCA/Ⅰ human lung adenocarcinoma cells and matrine conbined with anti-tumor drugs shows a significant synergistic effect on tumor cells.更多还原