【摘要】 利用Charleston ×东农594重组自交系构建的SSR遗传图谱,及混合线性模型方法对2002年到2006年连续5年的大豆油分含量进行QTL定位,并作加性效应,加性×加性上位互作效应及环境互作效应分析。共检测到11个控制油分含量的QTL,分别位于第A1、A2、B1、C2、D1a、D1b、F、H和O连锁群上,其中2个表现为遗传正效应,9个表现为遗传负效应,另检测到15对影响油分含量的加性×加性上位互作效应的QTL,解释该性状总变异的17.84%。发现9个QTL与环境存在互作,贡献率达到5.76%。更多还原

【Abstract】 Soybean [Glycine max (L.) Merr., 2n=2x=40] is grown worldwide, especially in the United States, Brazil, Argentina, and China. Increasing oil content in soybean seeds is one of the main aims in soybean breeding. Oil content is quantitative traits controlled by multiple genes. Many researches have used molecular markers to map quantitative trait loci (QTL). Currently, Soy- Base (2007) contains at least 68 QTL associated with oil content that have been mapped in many different populations and envi- ronments. The development of molecular tools has facilitated the task of identifying chromosomal regions related to particular traits. Song et al. (2004) developed an integrated genetic map spanning 2 523.6 cM across 20 linkage groups that contained 1 849 markers, including 1 015 SSRs, 709 RFLPs, 73 RAPDs, 24 classical traits, 6 AFLPs, 10 isozymes, and 12 others. In MAS, it would be more desirable to use confirmed QTL. For this purpose, it is necessary to conduct QTL, mapping studies in as many and as diverse environments and population as feasible. In this study, QTL of soybean oil content in five different years was analyzed with a recombination inbred lines (RIL) population derived from a cross between Charleston and Dongnong 594 by mixed linear model approach. 11 QTL with additive effects for oil content were mapped in the linkage groups A1, A2, B1, C2, D1a, D1b, F, H, and O. 15 QTL pairs with epistatic effects for oil content in the RIL were detected, and the general phenotypic variation was 17.84%. 9 QTL were detected from QTL× environment interaction, and the general contribution was 5.76%.更多还原