【摘要】 【目的】研究海藻糖(TH)和牛血清白蛋白(BSA)单独或配合使用对牛精液冷冻效果的影响。【方法】向种公牛精液商业稀释液中添加不同浓度的TH(0,0.000 37,0.025和0.05 mol/L)和BSA(0,1,2,3 g/L),TH和BSA的4个添加水平分别两两组合,组成16个处理,以其中二者添加水平均为0作为对照,制作0.25 mL细管冻精,解冻(40℃,10 s)后比较分析精子活率、顶体完整率和质膜完整性3个指标。【结果】单独添加TH、BSA及其互作对牛精液的冷冻效果与对照组相比达到显著水平。与对照组相比,单独添加不同水平的TH或BSA对冻后牛精子活率和质膜完整率均有明显影响(P<0.05),但对顶体完整率均影响不大(P>0.05),TH适宜的单独添加量为0.025mol/L,BSA为1 g/L;两者的交互作用对冻后牛精子活率、顶体完整率和质膜完整率均有明显影响(P<0.05),TH0.000 37 mol/L×BSA 2 g/L为较适宜的添加量。【结论】单独添加TH和BSA,均能对冻后精子品质产生明显影响;各TH×BSA组表现出显著的互作效应,其中以TH 0.000 37 mol/L×BSA 2 g/L的添加效果最好。更多还原

【Abstract】 【Objective】 The research was to study effects of trehalose and BSA on bull sperm cryopreservation.【Method】 Trehalose and bovine serum albumin were added into commercial sperm extender according to TH(0,0.000 37,0.025,0.05 mol/L)×BSA(0,1,2,3 g/L) content respectively.Samples were frozen in 0.25 mL straws.The frozen straws were thawed by immersing the straws in a water bath at 40 ℃ for 10 s.Sperm mortality,acrosome integrity and membrane integrity were analyzed and compared.【Result】 The addition of trehalose or bovine serum albumin of different levels alone can offer better(P<0.05)cryopreservation effect on frozen-thawed bull sperm mortality and membrane integrity when there were no obvious improvement(P>0.05) on sperm acrosome integrity and their best concentration was 0.025 mol/L and 1 g/L respectively.The treatment TH(0.000 37 mol/L)×BSA(2 g/L) is the best one in sixteen groups.【Conclusion】 Trehalose and bovine serum albumin can significantly improve(P<0.05)the quality of frozen-thawed bull semen.The interaction effects of trehalose and bovine serum albumin on cryopreserved bull sperm was observed.The treatment TH(0.000 37 mol/L)×BSA(2 g/L) is the best one.更多还原