【摘要】 【目的】研究减蛋综合征病毒(EDSV)河南HN03株纤维蛋白基因全序列,为进一步分析EDSV纤维蛋白基因的结构、功能以及EDSV基因工程疫苗的研究奠定基础。【方法】根据GenBank中收录的EDSV纤维蛋白基因序列(Z86065),设计并合成了1对引物,PCR扩增EDSV河南HN03株纤维蛋白基因。将扩增产物克隆入pTG19-T载体并测序。【结果】测序结果表明,HN03株纤维蛋白基因为1935 bp,包含一个完整的开放阅读框,编码644个氨基酸,推导的氨基酸序列有5个潜在的N-糖基化位点,有5个与二硫键形成有关的半胱氨酸。序列分析表明,EDSV HN03株与EDSV国际标准株AV-127纤维蛋白基因核苷酸同源性为99.3%,氨基酸同源性为99.2%;与鹌鹑腺病毒QU株纤维蛋白基因核苷酸同源性为98.9%,氨基酸同源性为98.4%。【结论】腺病毒纤维蛋白基因相当保守。更多还原

【Abstract】 【Objective】 The research is to study the full length nucleic acid sequence of fiber gene of Henan HN03 isolate of egg drop syndrome virus(EDSV).【Method】 One pair of primers was designed and synthesized according to the fiber gene nucleic acid sequence(Z86065) of(EDSV) retrieved from the GenBank.Fiber gene of Henan HN03 isolate of EDSV was amplified by PCR,then was cloned into pTG19-T Easy vector and sequenced.【Result】 The result indicated that the full length sequence of fiber gene consisted of 1 935 bp,which included one open-reading frame,encoding 644 amino acid residues.There were five potential N-glycosalation sites and five cysteines in the deduced amino acid sequence.The sequence analysis showed that the identity was 99.3% at nucleotide level between HN03 isolate and EDSV AV-127 strain,and 98.9%at nucleotide level between HN03 isolate and quail adenovirus QU strain.【Conclusion】 It showed fiber gene for adenovirus was highly conserved.更多还原