【摘要】 【目的】为建立醋酸甲羟孕酮(MPA)快速准确的免疫学分析技术,对MPA人工抗原的合成鉴定进行研究。【方法】将MPA肟化后与琥珀酸酐反应制得MPA半琥珀酸(MPA-HS),然后通过碳二亚胺法将MPA-HS与牛血清白蛋白(BSA)偶联合成人工抗原MPA-BSA,并采用紫外全波长扫描法、SDS-PAGE法、ELISA检测法和动物免疫试验对其进行鉴定。【结果】MPA与BSA的偶联比为8∶1时,合成的人工抗原MPA-BSA具有良好免疫原性与反应原性。【结论】成功合成了人工抗原MPA-BSA,为进一步的MPA免疫学分析技术研究奠定了基础。更多还原

【Abstract】 【Objective】 This study was to develop a rapid and sensitive technology for the detection of Medroxyprogesterone Acetate(MPA).The atrificial antigen of MPA was synthesized and identified in the study.【Method】 MPA hemi hemi-succinate(MPA-HS) was produced by the oximation reaction of MPA with the reaction between oximinooximated-MPA and with succinic anhydride,then the hapten MPA-HS was conjugated to protein carrier BSA by using the coupling agent diisopropylcarbodiimide,and the synthesis synthesized antigen MPA-BSA was identified by full-wavelength the ultraviolet full wavelength scanner,SDS-PAGE,ELISA test and animal-inoculation immunization test.【Result】 The results showed that the synthesis synthesized antigen MPA-BSA with good immunogenicity and reactogenicityantigenicity,and the optimized conjugation molecular ratio of coupling reaction was determined to be 8∶1.【Conclusion】 The synthesis of artificial antigen MPA-BSA set basis for the study of the immunology detection technology of MPA.更多还原