【摘要】 以犬瘟热病毒水貂分离株RNA为模板,经RT-PCR反应,扩增出融合蛋白基因的1053bp DNA片段,通过T-A克隆技术,成功构建克隆载体PMD-18T/F。序列分析表明:分离株与01-2689株、2544-Han95株、A75-17株、DOGDK91C株、00-2601株、ONP株、PDV-2株核苷酸序列同源性分别为94.3%、93.5%、94.4%、93.6%9、5.3%、97.3%和94.5%;氨基酸序列同源性分别为97.2%、96.6%、97.4%、97.4%、97.4%、97.2%和98.0%。抗原指数分析表明分离株与疫苗ONP株、强毒A75-17株在40-50位氨基酸间存在明显差异。更多还原

【Abstract】 The genomic RNA was extracted from isolated strain.The 1053bp DNA segment of fusion protein(F) gene was amplified by reverse transcriptase polymerase chain reaction(RT-PCR).The clone vector PMD-18T-CDVSJ F was constructed successfully by T-A clone technique.The sequence of the fusion gene and deduced amino acid of isolated strain showed an identity of 94.3%,93.5%,94.4%,93.6%,95.3%,97.3%,94.5% and 97.2%,96.6%,97.4%,97.4%,97.4%,97.2%,98.0% with 01-2689,2544Han95,A75-17,DOGDK91C,00-2601,ONP,PDV-2,respectively.Antigenicity analysis showed that isolated strain was different from ONP and A75-17 in 40-50th amino acid antigenicity.更多还原