【摘要】 目的:制备兔抗人转录因子TFDP3、TFDP1的多克隆抗体,并鉴定其反应性及特异性。方法:选择TFDP3和TFDP1差异较大肽段,利用人工合成多肽免疫家兔,采用真核表达载体瞬时转染HEK293细胞表达TFDP3和TFDP1真核蛋白,通过Western blot分析抗体的反应性和特异性。结果:通过氨基酸序列比对选择TFDP3 17-26,TFDP1 17-32氨基酸残基合成多肽,制备了兔抗人TFDP3、TFDP1多肽抗体,Western blot检测显示,抗TFDP3抗体可以特异性识别TFDP3,而抗TFDP1抗体既可识别TFDP1又可与TFDP3结合。结论:制备了兔抗人TFD31和TFDP1的多肽抗体,且TFDP3抗体与TFDP1无交叉反应。更多还原

【Abstract】 AIM: To prepare the polyclonol antibody against human transcriptional factors DP3(TFDP3) and DP1(TFDP1) and identify their reactivity and specificity.METHODS: Two totally different peptides between TFDP1 and TFDP3 were chosen as antigens to inoculate rabbits.The proteins from HEK293 cells were transiently transfcted with eukaryotic expression vectors of TFDP1 and TFDP3.The reactivity and specificity of antibodies were detected by Western blot.RESULTS: The amino acid residues of TFDP3 17-26 and TFDP1 17-32 were selected and synthesized to produce polypeptide antibodies.Western blot analysis demonstrated that anti-TFDP3 antibody could specifically recognize TFDP3 protein,while anti-TFDP1 antibody could both recognize TFDP1 and bind TFDP3 protein.CONCLUSION: The polypeptide antibodies against TFDP3 and TFDP1 have been prepared.The anti-TFDP3 antibody has no cross-reaction with TFDP1.更多还原