【摘要】 目的:构建弓形虫多表位DNA疫苗并研究其免疫保护效果。方法:将编码含弓形虫多个T、B细胞表位的6段弓形虫多肽基因,以5个甘氨酸编码基因相间隔相连接,克隆入真核表达质粒pcDNA3.1(+)中,构建成多表位弓形虫DNA疫苗。免疫BALB/c小鼠,测定其诱导的特异性抗体水平及T淋巴细胞增殖状况,同时进行弓形虫攻击感染保护实验。结果:成功构建了包含多个弓形虫表位的真核表达质粒pcDNA3.1/T-ME,以其作为DNA疫苗免疫小鼠,可诱导机体产生弓形虫特异性的体液及细胞免疫应答,产生有效的抗弓形虫保护性免疫应答。结论:构建的弓形虫多表位DNA疫苗能诱导机体产生有效的保护性免疫应答,在控制弓形虫感染上具有可行性。更多还原

【Abstract】 AIM:To construct multi-epitope DNA vaccine for Toxoplasma gondii and study its protective immunity response.METHODS:The gene encoding six polypeptides of T.gondii,which consists of plenty of T and B epitopes,was cloned into the eucaryotic expression vector pcDNA3.1(+).BALB/c mice were vaccinated by this multi-epitope based DNA vaccine(intramuscular needle injection).The specific antibody and T cell proliferation were determined.Meanwhile,the DNA-vaccinated mice were challenged with a lethal dose of T.gondii tachyzoites for further observation.RESULTS:The eukaryotic expression plasmid(pcDNA3.1/T-ME)encoding plenty of T.gondii epitopes was constructed successfully.pcDNA3.1/T-ME immunization induced T.gondii specific humoral and cellular immunity in mice.The mice immunized with pcDNA3.1/T-ME survived significantly longer than the mice in control after challenged by T.gondii RH strain infection.CONCLUSION:The multi-epitope DNA vaccine can induce the protective immunity against T.gondii infection effectively in vivo,which is a potential strategy to control T.gondii infection.更多还原