【摘要】 目的:探讨γδT细胞在过敏性支气管哮喘发病机制中的作用。方法:来自门诊的40例粉尘螨和屋尘螨点刺阳性、部分控制过敏性支气管哮喘患者随机分为常规治疗组(A组)和吸入减敏组(B组),每组各20例。所有患者接受相同的基础治疗,常规治疗组雾化吸入生理盐水5mL、吸入减敏组雾化吸入特异性变应原,每周2次,持续6个月。治疗前后用流式细胞术(FCM)检测外周血及诱导痰中γδT细胞占淋巴细胞百分比;IL-4+、IFN-γ+γδT细胞占淋巴细胞百分比。结果:治疗后B组外周血、诱导痰中γδT细胞占淋巴细胞百分比低于治疗前;B组外周血和诱导痰中IFN-γ+γδT细胞占淋巴细胞百分比增高。结论:特异性变应原雾化吸入减敏疗法有效防治哮喘可能与γδT细胞有关;γδT细胞可能参与哮喘发病机制。更多还原

【Abstract】 AIM:To explore the role of γδT cells in the pathogenesis of allergic bronchial asthma.METHODS:Forty patients with Der.p and Der f allergy and partly-controlled asthma were involved in a random study.Besides the fact that all patients were treated with same basic treatment.Patients in group A were nebulized with 5 mL normal saline while group B with specific allergen twice a week for six months.The percentage of γδT cells in peripheral blood and induced sputum and the percentage of IL-4+ and IFN-γ+ γδT cells were detected by flow cytometry(FCM)before and after treatment.RESULTS:The percentage of γδT cells in group B decreased after treatment,and the percentage of IFN-γ+ γδT cells in both peripheral blood and induced sputum increased significantly in group B than that in group A.CONCLUSION:γδT cells,which may participate in the pathogenesis of bronchial asthma,may contribute to the prevention and treatment of bronchial asthma by desensitization of allergen nebulization.更多还原