【摘要】 目的对绞股蓝属(Gynostemma BL.)6种植物的遗传多样性与亲缘关系进行分析。方法利用ISSR标记技术对57条引物进行初筛,选择其中适合的11条引物对材料进行遗传多样性分析,并根据遗传距离构建UPGMA聚类图。结果11个ISSR引物共检测到120个位点,平均每个引物检测到10.91个位点,多态性比率(PPB)达到98.33%,6个物种的多态性比率分布于34.17%~1.67%之间,Shannon信息指数(I)为0.4145,Nei′s基因多样性指数(He)为0.270 7,G.pen-taphyllum,G.cardiospermum,G.longipes,G.yixingense,G.laxiflorum的分类处理与形态学研究结果基本一致,G.pentagynum与G.longipes遗传距离最近。结论ISSR分子标记适合于绞股蓝属植物遗传多样性研究。更多还原

【Abstract】 Aim To investigate the genetic diversity and affinity among 6 species of Gynostemma BL.by inter-simple sequence repeat(ISSR)technique.Methods 11 appropriate ISSR primers were selected from a total of 57 ISSR ones for PCR amplification.Results 120 bands were amplified by 11 informative and reliable primers,with 10.91 bands per primer in average,the rate of polymorphic bands was 98.33%,the percentages of the polymorphic sites in the 6 species differed within 34.17%～1.67%,a high level of genetic diversity in Gynostemma BL.was revealed that Shannnon′s gene diversity(I) was 0.414 5,Nei′s gene diversity(He) was 0.270 7.The UPGMA cluster analysis indicated that the genetic relationship was basically consistent with the conventional classification,G.pentagynum and G.longipes had the smallest genetic difference.Conclusion The results reveal that ISSR method is suitable for genetic diversity analysis of Gynostemma BL.更多还原