【摘要】 目的:探讨IFN-γ基因多态性与HBV感染不同临床转归之间的相关性。方法:应用聚合酶链反应-序列特异性引物法(PCR-SSP)检测60例自限性HBV感染者(SR组)和118例慢性持续性HBV感染者[包括无症状HBV携带者(AsC组)36例和进展性肝病者82例(慢性乙型肝炎50例、乙肝后肝硬化32例)]的IFN-γ基因+874T→A、+2109A→G两个位点的多态性,比较各组间基因型和等位基因频率。结果:①+874T→A位点多态性中,慢性持续性感染者总AA基因型频率比SR组显著升高(P<0.05),差异有显著性。等位基因A的频率,进展性肝病组均显著高于SR组(P<0.05)。②+2109A→G位点多态性中,进展性肝病组总GG基因型显著高于SR组(P<0.05),而肝硬化和慢乙肝组之间,SR和AsC组之间差别无显著性。结论:IFN-γ基因+874T→A、+2109A→G两个位点的多态性,与HBV感染不同结局之间有关,携带+874T→AAA基因型和A等位基因的HBV感染者容易发展为慢性,A等位基因更趋向于进展性肝病,而携带+2109A→GGG基因型患者则容易进展为慢性乙型肝炎甚至肝硬化。更多还原

【Abstract】 Objective:To explore the relativity between IFN-γ gene polymorphism and the clinical outcomes of HBV infection.Methods:A case-control study and PCR-SSP was used to detect IFN-γ+874 T→A and +2109 A→G polymorphism of 118 chronic HBV infected patients and 60 subjects which spontaneously recovered from HBV infection(SR group).The chronic HBV infection group included 36 asymptomatic HBV carriers(AsC group),50 chronic hepatitis B and 32 HBVrelated liver cirrhosis.Results:The AA genotype frequency of IFN-γ+874 T→A was significantly higher in chronic HBV infection group than that of SR grou(pP<0.05).The frequency of A allele was significantly higher in all advanced liver disease group(including chronic hepatitis B and liver cirrhosis) than that of SR group( P<0.05).The GG genotype frequency of IFN-γ+2109 A→G was significantly higher in all advanced liver disease group than that of SR group(P<0.05).Conclusion:The results suggest that IFN-γ+874 T→A AA genotype and A allele may be associated with susceptibility of chronic HBV infection.The A allele may be related with advanced liver diseases.IFN-γ+2109 A→G GG genotype may be related with susceptibility of chronic hepatitis B,even liver cirrhosis.更多还原