【摘要】 为探讨γ-氨基丁酸(GABA)在植物生长发育中的调控机理,以含有3种不同浓度的GABA的1/2MS培养基培养烟草(Nicotiana tabacum)品种‘SR1’种子,结果表明:低浓度GABA(1mmol/L)促进了烟草幼苗的生长,主根的长度比对照高30%,同时该浓度处理提高了MAPK基因的表达水平;较高浓度(10~100mmol/L)GABA抑制了主根的伸长,表现出乙烯反应的效应。在叶和根中,ACS1基因表达水平的提高受不同浓度GABA的调节。MAPK和ACS1基因对GABA信号的不同表达模式可能与不同的信号途径有关。更多还原

【Abstract】 To understand the mechanism of γ-aminobutyric acid (GABA) in plant growth and development,the seedlings of Nicotiana tabacum (SR1) were cultured in 1/2 MS medium plus three different GABA concentrations.The results showed that GABA can stimulate the growth of Nicotiana tabacum seedlings at a low concentration (1mmol/L),and the length of primary roots is significant longer than that of the control (higher about 30%);The expression of MAPK was promoted under the treatment of a low GABA concentration (1mmol/L).However,the elongation of primary roots was inhibited at a high GABA concentration (10-100 mmol/L),demonstrating the typical feature of ethylene response effect.The expression of ACS1 was enhanced by different GABA concentration,100 mmol/L in leaves and 10 mmol/L in roots.Different expression of MAPK and ACS1 to GABA signals may be involved different signal transduction pathway.更多还原