【摘要】 【目的】建立龙血竭中新化合物-剑叶龙血素C的分离与测定含量方法。【方法】采用PhenomenexC18(250 mm×4.6 mm,5μm)色谱柱,甲醇-水(75:25,v/v)为流动相,流速为1 m/min,检测波长为295nm,柱温50℃;用外标法定量。【结果】剑叶龙血素C在27.25~327 ng范围有良好线性关系,r=0.9997,理论塔板数以剑叶龙血素C计为4500;方法回收率为96.90%,RSD为1.2%。【结论】本方法是测定龙血竭中剑叶龙血素含量的准确的定量方法。更多还原

【Abstract】 【Objective】To establish a HPLC method for the separation and determination of Cochinchinenin C in Dragon’s Blood.【Methods】The chromatographic system consisted of Phenomenex C18(250 mm×4.6 mm,5 μm) column and mobile phase of CH3OH-H2O(75∶25) and detected at 295 nm,the temperature of column was 50℃ and quantitating with external standard method.【Results】 The standard curves of Cochinchinenin C was linear in the concentration range of 27.25～327 ng(r=0.9997).The theoretical plate number is 4500.The average recovery rate was 96.90%(RSD=1.2%).【Conclusions】The established method is accurate,sensitive,simple and reliable for determination of Cochinchinenin C in Dragon’s Blood.更多还原