【摘要】 【目的】植物病原真菌毒素是一类重要的微生物源除草剂,本研究旨在找到一个新的具有除草活性的化合物结构。【方法】在前期薄层层析法、柱层析法和高效液相色谱法分析的基础上对灰葡萄孢诱变菌株BC7–3的代谢产物中具有除草活性的5个不同组分分别进行了液相色谱制备。【结果】本研究得到了一个纯度达99.38%对单子叶杂草马唐具有较强杀除活性的纯组分,通过对纯组分的物理性状测定并结合紫外–可见吸收光谱、红外光谱以及核磁共振波谱等分析方法鉴定化学结构为10–顺–二氢化灰霉二醛。【结论】研究的结果为微生物除草剂的创新和开发奠定了理论基础。更多还原

【Abstract】 [Objective] Toxin produced by phytopathogenic fungi is one of the important microbial herbicides. We found a new compound with herbicidal activity. [Methods] Five different ultraviolet absorption components were isolated from the filtrate of Botrytis cinerea isolate 7–3 culture. [Results] Of the five components, one showed strong inhibitory to Digitaria sanguinalis. The pure fraction with high herbicidal activity was obtained by HPLC purification. Its structure was identified as 10–syn–dihydrobotrydial by Ultraviolet and Visible Spectroscopy, Infrared Spectrum, Nuclear Magnetic Resonance Spectroscopy analysis. [Conclusion] The findings are important for future preparation and application of the herbicide.更多还原