【摘要】 【目的】研究单级自养脱氮系统中亚硝化菌株的培养及代谢特征,为系统运行操控提出理论指导。【方法】从单级自养脱氮系统活性污泥中采集微生物样品,经过4次富集和分离过程,最终获得一株亚硝化能力较强的菌株N1,通过显微镜观察及16S rDNA序列分析鉴定该菌株,研究摇瓶装量、pH、温度及底物浓度对其代谢过程的影响。【结果】该菌株与Nitrosomonas sp.NL7(AY958677)、Nitrosomonas AS1(EF016119)、Nitrosomonas sp.Is32(AJ621027)相似性分别为97%、96%和96%,对氧气需求量存在较严格要求,pH及温度对其氨氧化活性具有明显的影响,最适条件分别为pH8.0和30℃,在氨氮浓度80～800mg/L较宽的底物浓度范围内具有活性,当氨氮浓度高达800mg/L时,其氨氧化活性没有受到明显的抑制。【结论】该N1菌株为亚硝化单胞菌(Nitrosomonas sp.),与已有报道的其它亚硝化菌相比,N1对氨氮有较强降解能力及较广的浓度适应范围。更多还原

【Abstract】 Objective To study the characteristics of the Nitroso-bacteria in one-step completely autotrophic nitrogen removal process. Methods We took samples from activated sludge of the one-step completely autotrophic nitrogen re-moval reactor. Through four times of enrichments and isolations,one Nitroso-bacteria named N1 was obtained. We iden-tified this strain by microscope and 16S rDNA analysis and studied the effect of pH,temperature as well as ammonium concentration on the metabolism of N1. Results Of the N1 nucleotides,97%,96% and 96% were identical with the conserved fragment of Nitrosomonas sp. NL7 (AY958677),Nitrosomonas AS1 (EF016119) and Nitrosomonas sp. Is32 (AJ621027) respectively. The optimal temperature and pH of N1 were 8.0 and 30℃ and sufficient dissolved oxygen was demanded. In addition,no restraint effect was turned up in the ammonium concentration of 80-800 mg/L. Conclusion N1 was identified as Nitrosomonas sp. Compared with the relative references,this strain was ammonium-tolerant.更多还原