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利用门多萨假单胞菌NK-01合成中长链聚羟基脂肪酸酯(PHA_MCL)

Synthesis of Medium-chain-length Polyhydroxyalkanoate (PHA_MCL) by Pseudomonas mendocina NK-01

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【Author】 TAO Jian1,2 ZHENG Cheng-Gang1 ZHANG Bin1 GUO Wen-Bin1 WANG Shu-Fang2 SONG Cun-Jiang1,2 (1. The Key Laboratory of Molecular Microbiology and Technology, Ministry of Education, Nankai University, Tianjin 300071) (2. The Key Laboratory of Bioactive Materials, Ministry of Education, Nankai University, Tianjin 300071)

【机构】南开大学生命科学学院分子微生物学与技术教育部重点实验室；南开大学生命科学学院生物活性材料教育部重点实验室；南开大学生命科学学院分子微生物学与技术教育部重点实验室天津300071南开大学生命科学学院生物活性材料教育部重点实验室天津300071；天津300071；天津300071南开大学生命科学学院生物活性材料教育部重点实验室天津300071；

【摘要】从土壤中分离到1株能够合成中长链聚羟基脂肪酸酯(PHAMCL)的细菌,经分类鉴定命名为Pseudomonas mendocina NK-01。对于该菌株在氮源限制的情况下,利用葡萄糖合成的PHAMCL进行了结构分析。同时,获得了该菌株PHA合成酶基因phaC1,利用大肠杆菌表达载体pBluescript SK-构建了重组载体pBSphaC1,并在E.coli JM109中成功表达。以所构建的工程菌株进行了发酵合成。更多还原

【Abstract】 A strain which can synthesize medium-chain-length polyhydroxyalkanoate (PHAMCL) was isolated from soil. It was named as Pseudomonas mendocina NK-01 through classification and identification. The PHAMCL was synthesized by P. mendocina NK-01 from glucose in the condition of limited nitrogen source. The structure of it was characterized. In order to construct a recombined plasmid pBSphaC1, an expressing plasmid of E.coli, after phaC1 gene encoding PHA synthase was cloned from Pseudomonas mendocina NK-01 by PCR amplification, it was linked into pBluescript SK—. PhaC1 gene was expressed successfully in E.coli JM109. Fermentation synthesis was also investigated by recombined E.coli.更多还原