【摘要】 对以DL-2-氨基-Δ~2-噻唑啉-4-羧酸(DL-2-amino-Δ~2-thiazoline-4-carboxylic acid,DL-ATC)为底物原料,经微生物酶法催化合成L-半胱氨酸,并进一步氧化和分离纯化产物L-胱氨酸的生产工艺和条件进行了研究。建立了以恶臭假单胞菌TS1138(Pseudomonas putida TS1138)全细胞为酶源,反复多次催化底物合成L-半胱氨酸,并以2.0%二甲基亚砜(DMSO)为氧化剂氧化生成L-胱氨酸,进而通过001×7型阳离子交换树脂纯化胱氨酸的新工艺。采用高效液相色谱法考察该方法L-胱氨酸的总收率可以达到78.55%,纯度为99.12%。该方法简单高效,解决了酶稳定性差不能重复使用,而固定化酶方法繁琐成本高的问题,为我国L-半胱氨酸和L-胱氨酸的生产开辟一条新途径。更多还原

【Abstract】 A technology of L-cystine production was studied in this paper,which included microbial enzymatic conversion of DL-2-amino-Δ~2-thiazoline-4-carboxylic acid(DL-ATC)to L-cysteine,subsequent oxidization of L-cysteine to L-cystine and its purification.The cells of Pseudomonas putida TS1138 could be repetitively used as the enzyme sources to convert the substrate DL-ATC to L-cysteine.After being oxidated by 2%dimethy-sulforide(DMSO),L-cystine could be harvested and further purified by the positive ion-exchange resin 001×7.High Performance Liquid Chromatography(HPLC)identified the purified L-cystine as having a total recovery of 78.55%and purity of 99.12%.This study demonstrated an efficient and convenient method for L-cystine production,which overcame the instability of enzymes,troublesome procedures and high cost of enzyme immobilization as contrasted to the traditional method.All in all,it provides a new approach for industrial production of L-cystine as well as L-cysteine.更多还原