【摘要】 从多管藻和螺旋藻中提取了藻胆蛋白,利用藻胆蛋白在可见光区具有吸收的特点,建立了藻胆蛋白含量的快速测定方法.在波长565和620 nm处测定藻红蛋白和藻蓝蛋白在0—1.2 mg/mL内具有良好的线性关系,r藻红=0.9995,r藻蓝=0.9998,该方法测定藻红、藻蓝蛋白的回收率分别为99.07%和99.11%.与传统的Lowry法相比,该方法测定蛋白质简便快速,重现性好.更多还原

【Abstract】 The phycoerythrin is extracted from Polysiphonia urceolata and Spirulina platensis.A simple and rapid method for the determination of phycoerythrin content is established based on the adsorption of R-phycoerythrin(R-PE) and C-phycocyanin(C-PC) under visible light area.The absorbance value at 565nm and 620nm had a good linearity with the mass concentration of R-PE and C-PC in the range 0—1.2mg/mL,with rR-PE=0.9995 and rC-PC=0.9998.The recovery ratio for R-PE and C-PC is 99.07% and 99.11%,respectively.This determination method has a good reproducibility,which is simpler and rapider than the conditional detection-Lowry method.更多还原