【摘要】 从广西某含硫温泉中分离得到了嗜酸氧化硫硫杆菌菌株,经分离纯化与初步培养后进行浸出低品位磷矿实验。其后,利用微波诱变的方式对该细菌进行了诱变育种研究。结果表明:经分离纯化得到的At t菌对磷矿矿样有一定的浸出能力,但效果不佳;微波辐照能有效引起At t菌发生突变,使其生长活性及产酸能力均发生改变,与此对应的是诱变后的At t菌菌株对磷矿矿样的浸出能力较原菌株有了明显的改变;在不同诱变时间下得到菌株的浸矿能力有所不同,其中诱变10 s的菌株对磷矿的浸出率高于其它菌株,浸矿20 d后浸出率达到32.74%,比原菌株的浸出率提高了42%。更多还原

【Abstract】 A strain of Acidithiobacillus thiooxidans(At t) isolated from a sulfur-containing hot spring in Guangxi was used to leach low-grade phosphate ore after being purified and cultivated.Then microwave mutagenesis study was carried out with the strain.The isolated and purified strain of At t had the capability of leaching low-grade phosphate ore,but the leaching-rate was low.Microwave could cause mutation of At t effectively and change its growth activity and acid-producing capacity.Strains treated for different mutagenesis time showed different bioleaching ability.The leaching-rate of the strain mutagenized with microwave for 10 s was the highest and reached 32.74% after bioleaching for 20 d,which increased almost 42% than that of the original At t strain.更多还原