【摘要】 试验从连续流发酵产氢反应器（ZL92114474.1）中分离筛选出一株高效纤维素降解产氢细菌Clostridium sp.X9。X9利用微晶纤维素（MC）作为发酵产氢底物,得到最大单位体积产氢量(Y_H2)、比产氢率(Y_H2/s)和纤维索降解率分别为780 mL H₂/L-culture、5.1 mmol H₂/g-cellulose和69.6%。采用酸、碱、氨水和酸化汽爆方式预处理玉米秸秆,结果表明,酸化汽爆方式可以获得最佳的预处理效果。X9利用酸化汽爆玉米秸秆（cSES）发酵产氢的Y_H2、Y_H2/s和纤维素降解率分别达到730 mL H₂/L-culture、4.3mmol H₂/g-cellulose和64%。这说明新菌种X9在利用玉米秸秆类生物质纤维素发酵产氢方面具有很好的应用潜力。更多还原

【Abstract】 In this study,Cellulolyticum sp.X9,anaerobic high efficiency cellulose degrading and hydrogen producing bacterium,was isolated from a continuous-flow H₂ producing reactor（ZL92114474.1）.Experimental results showed that strain X9 has high hydrogen yield(Y_H2)of 780 ml H₂/L-culture,specific H₂ production rate(Y_H2/s)d 5.1 mmol H₂/ g-cellulose and cellulose degradation degree of 69.6% feeding with microcrystalline cellulose（MC）.Then,strain X9 was adopted to investigate H₂ production capabilities directly from corn stalks fermentation following with four different pretreatment methods.Of which,steam explosion pretreatment showed the highest H₂ yield(Y_H2)and corn stalk degra- dation degree.This indicated that Cellulolyticum sp.X9 has great potential for fermentative H₂ production from lignocel- lulose biomass like corn stalks after effective pretreatment.更多还原