【摘要】 采用氯胺-T法制备了¹²⁵I-HSA-IFN-α2b,标记率为82.7%,放化纯度>95%,放射性比活度为O.26 TBq/g。体外WISH/VSV系统抗病毒活性分析表明,¹²⁵I标记对HSA-IFN-β2b的生物学活性几乎无影响。¹²⁵I-HSA-IFN-α2b的血清和各组织的回收率分别为102.0%、104.0%（心脏）、100.4%（肺）、100.9% （肝）、104.1%（脾）、101.4%（肾）和103.0%（小肠）,均符合方法学考核中回收率达90%～110%的要求。¹²⁵I-HSA-IFN-α2b的溶液干扰、血清样品干扰和组织样品干扰实验的结果表明,溶液、血清和组织样品对测定均无干扰,可以直接进行测定。根据实验结果可以初步推断,采用¹²⁵I-HSA-IFN-α2b同位素示踪法进行HSA-IFN-α2b的体内分布排泄研究是可行的。更多还原

【Abstract】 ¹²⁵I-HSA-IFN-α2b was prepared with the method of CH-T.The labelling yields was 82.72%,radiochemical purity was more than 95% and the radioactivity was 0.26 TBq/ g.The activity of antivirus of ¹²⁵I-HSA-IFN-α2b was compared with that of HSA-IFN-α2b by WISH/VSV system in vitro.The results showed that bioactivity of HSA-IFN-α2b had no change before and after being labelled.The serum recovery 102.0% and organ recovery 104.0%（heart）,100.4%（lung）,100.9%（liver）,104.1%（spleen）,101.4%（kidney）, 103.0%（small intestine）of ¹²⁵I-HSA-IFN-α2b met the requirements.The results of the in- terference experiments showed that there was no interference on the determination.There- fore,¹²⁵I-HSA-IFN-α2b can be used in studying biodistribution and excretion of HSA-IFN-α2b.更多还原