【摘要】 目的构建可用于大肠杆菌表达系统的含变形链球菌唾液结合区段(SBR)基因的表达载体pcMVT7-SBR。方法用定向克隆方法将SBR基因插入表达载体pcMVT7,构建重组原核表达质粒pcMVT7-SBR。结果经酶切鉴定及DNA序列测定,重组质粒pcMVT7-SBR序列及阅读框架正确。结论SBR表达载体构建成功,为防龋疫苗的动物实验研究奠定基础。更多还原

【Abstract】 Objective: To obtain the prokaryotic expression vector containing the saliva binding region(SBR) gene of Streptococcus mutans.Methods: By directional cloning method,SBR gene fragment was cloned into the expression vector pcMVT7 and recombinant prokaryotic expression plasmid pcMVT7-SBR with right reading frame was successfully constructed.Results: Restriction endonuclease and DNA sequencing techniques were used to identify the recombinant plasmid DNA.The results were in correspondence with the initial design.Conclusion: The recombinant expression plasmid pcMVT7-SBR is constructed successfully,which lays a good foundation for animal experiment of anti-caries vaccine.更多还原