【摘要】 选取规范方法、超声波法、反复冻融法、延时提取法、热丙酮法、丙酮加热法、热乙醇法、混合溶剂法8种常见的浮游植物叶绿素a的测定方法,以实验室培养的微囊藻(Microcystis aeruginosa)水华样品为研究对象进行叶绿素a含量的测定。从叶绿素a提取的完全性、测定数据的稳定性、试验操作的简便性等方面对8种方法进行评价,并对叶绿素a结果测量的单色法和三色法进行分析讨论。试验结果表明,选取的8种测定方法中,包括国家标准方法在内的大多数方法,存在操作耗时长、过程复杂、叶绿素提取不完全的缺点;而丙酮加热法测量叶绿素a的提取效率高、数据稳定性好,操作耗时短、简便,可推荐作为一种常用的浮游植物叶绿素a的测量方法;三色法和单色法测定方法的选取应综合考虑。更多还原

【Abstract】 This study compared eight methods for phytoplankton chlorophyll-a concentration measurement-national standard method,ultrasonic wave extracting method,freezing and melting method,extended extracting method,hot-acetone method,heating-acetone method hot-ethanol method,mix-solvent method.We choose Microcystis aeruginosa cultivated in the laboratory as the research object.Assess these methods from perfectibility,stability and convenience.Then analyze the methods of three-color spectrophotometry and single-color spectrophotometry.The results show that most of these methods,including the national standard method,are time-consuming,operation-perplexing and extracting-imperfectible.Whereas,heating-acetone method is extracting-perfectible,stable,time-saving and convenient.It can be recommended as a common methods for phytoplankton chlorophyll-a concentration measurement.methods of three-color spectrophotometry and single-color spectrophotometry should be considered synthetically when measuring.更多还原