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不同质量浓度氨氮对轮叶黑藻和穗花狐尾藻抗氧化酶系统的影响

Effects of different concentrations of NH4+ on antioxidant system of Hydrilla verticillata and Myriophyllum spicatum

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【作者】 金相灿郭俊秀许秋瑾扈学文张瑞玖

【Author】 Jin Xiangcan1, Guo Junxiu1,2, Xu Qiujin1, Hu Xuewen2, Zhang Ruijiu2 1. State Environmental Protection Key Laboratory for Lake Pollution Control//Research Center of Lake Eco-environment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China; 2. College of Agronomy , Inner Mongolia Agriculture University, Huhhot 010018, China

【机构】 中国环境科学研究院湖泊生态环境创新基地/国家环境保护湖泊污染控制重点实验室内蒙古农业大学农学院内蒙古农业大学农学院 北京100012北京100012内蒙古农业大学农学院内蒙古呼和浩特010018北京100012

【摘要】 在不同质量浓度(0、0.2、1.5、2.0、4.0、8.0mg·L-1)氨氮条件下,对沉水植物轮叶黑藻[Hydrilla Verticillata(L.f)Royle]和穗花狐尾藻[Myriophyllum spicatum L.]的蛋白质含量、丙二醛(MDA)含量、超氧化物歧化酶(SOD)及过氧化物酶(POD)活性变化进行了研究。实验结果显示,当氨氮质量浓度为0.2mg·L-1时,为轮叶黑藻的适宜生长质量浓度,表现为常用的膜脂过氧化指标MDA含量和SOD活性均变化比较平缓,且蛋白质含量增加,而穗花狐尾藻由酶学指标显示,氨氮质量浓度小于0.2mg·L-1时对它的正常生理代谢产生了影响;当氨氮质量浓度在1.5~4.0mg·L-1的范围则对轮叶黑藻产生胁迫作用,而穗花狐尾藻在此质量浓度范围能正常生长;当氨氮质量浓度为8.0mg·L-1时,高氨氮对2种沉水植物都表现出胁迫效应,且对轮叶黑藻的胁迫作用更强。实验结果表明在进行湖泊水生植物修复时,相对于轮叶黑藻,穗花狐尾藻更适合作为生态恢复的先锋物种。

【Abstract】 This paper investigated the contents of protein and MDA, peroxidase ( POD) and superoxide dismutase (SOD ) activities of Hydrilla verticillata and Myriophyllum spicatum in different concentrations of NH4+ (0, 0.2, 1.5, 2.0, 4.0 and 8.0 mg·L-1) culture. Results came out that the growth of H. verticillata was fitting at 0.2 mg·L-1 concentration of NH4+, the contents of MDA and SOD activities all relatively mild change, and the contents of protein were increased, but guide line of enzymology showed that M. spicatum was effected when the concentration of NH4+ were less than 0.2 mg·L-1. H. verticillata was stressed at 1.5 mg·L-1 to 4.0 mg·L-1 concentration of NH4+, but for M. spicatum, it vegetated naturally within the confines of the concentration . If treated with 8.0 concentration of NH4+, H. verticillata and M. spicatum all were stressed, and H. verticillata was stressed more seriously. It demonstrated that M. spicatum is suit for pioneer plant comparatively during the process of lake restoration.

【基金】 国家“973”项目(2002CB412307)
  • 【文献出处】 生态环境 ,Ecology and Environment , 编辑部邮箱 ,2008年01期
  • 【分类号】X173
  • 【被引频次】58
  • 【下载频次】716
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