【摘要】 采用热水浸提,乙醇沉淀的方法得到大马勃粗多糖,经Sevag法脱蛋白,DEAE-sepharose fast flow离子交换层析及sephacryl S-300HR凝胶过滤法得大马勃多糖(CGPⅠ-1)。气相色谱研究表明,其单糖组成为:Gal,Glc,Man等四种单糖,其中Gal,Glc,Man,摩尔比为3.92∶11.28∶1.22。经部分酸水解,红外光谱及核磁共振光谱,高碘酸氧化,Smith降解等分析,CGPⅠ-1的主链由Man和Glc构成,存在β型和α型两种糖苷键构型,支链或主链的末端残基由β-Gal(1→4),β-Glc(1→6),α-Glc(1→4)构成,其分子中存在酰胺结构。原子力显微镜观察发现CGPⅠ-1呈分支的线性分子,在水溶液中容易互相缠绕形成强大的网络结构。更多还原

【Abstract】 Calvatia geigantea polysaccharide CGP was extracted by hot water,precipitated by ethanol,and deproteinized by Sevag method.The CGPⅠ-1,a main constituent of CGP,was obtained by DEAE-sepharose and Sephacryl S-300HR gel-chromatography.The acid hydrolyzed product and GC-MS analysis of the CGPⅠ-1 showed that CGPⅠ-1 is composed of Gal,Glc,Man and so on.Gal,Glc and Man in a molar ratio of 3.9∶11.28∶1.22.IR analysis,partial hydrolysis with acid,periodate oxidation,Smith degradation,and 1H NMR,13C NMR analysis revealed that the main chain of CGPⅠ-1 is mainly made up of Man and Glc.The side chain or terminal residues is composed of β-Gal(1→4),β-Glc(1→6),α-Glc(1→4).There was amide group in CGPⅠ-1.The molecular structure of the CGPⅠ-1 at different concentrations was observed with atomic force microscopy(AFM).The CGPⅠ-1 molecule had a linear backbone with branches.The net work structure of CGPⅠ-1 was formed through entanglement between the molecules of the polysaccharide.更多还原