【摘要】 首次采用反相高效液相色谱法测定茜草根中1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]的含量。色谱柱为Purospher star RP C18色谱柱(250mm×4.6mm,5μm),流动相为甲醇:水:四氢呋喃(65:34.7:0.3),流速为1.0mL/min,检测波长为276nm,柱温为25℃。该方法的线性范围为0.020~0.160μg,r=0.9998,平均回收率为101.5%,RSD为2.0%(n=6)。该方法测定1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside]含量灵敏、准确、重现性好。更多还原

【Abstract】 To develop a HPLC method for the determination of 1,3,6-trihydroxy-2-methylanthra-quinone-3-O-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside] in Rubia.cordifolia for the first time.HPLC was carried out on a Purospher star RP C18 column(250 mm ×4.6 mm,5 μm) at 25 ℃ using MeOH-H2O-tetrahydropyrane (65:34.7:0.3) as mobile phase,at a flow rate of 1.0 mL/min.The detection wavelength was 276 nm.The linearity was found in the range of 0.020-0.160 μg,r=0.9998,the average recovery was 101.5%,RSD was 2.0%.This method is reliable,accurate and suitable for the determination of 1,3,6-trihydroxy-2-methylanthraquinone-3-O-[3-O-acetyl-α-L-rhamnopyranosyl-(1→2)-β-D-glucopyranoside].更多还原