【摘要】 目的:利用大鼠子宫内膜异位症模型,研究人重组干扰素α-2(bIFNα-2b)对大鼠子宫内膜异位症的影响,并比较不同给药方式的疗效。方法:将成模的大鼠随机分为实验组(An=15)、实验组(Bn=15)和对照组(n=8)3组,实验组A予人重组IFNα-2b10万U腹腔内注射1次,实验组B予皮下注射IFNα-2b10万U共3次,对照组不予处理。于用药后第4、6、8周开腹探查,观察移植物生长情况,免疫组化方法测定异位病灶内的血管内皮生长因子(VEGF)表达和微血管密度(MVD)。结果:实验组异位病灶体积呈持续减小的趋势,在治疗8周后2实验组病灶体积均明显小于对照组(P<0.05);实验组VEGF表达与MVD值都明显小于对照组(P<0.05)。2实验组间在治疗8周时病灶体积、VEGF表达和MVD值上差异无统计学意义(均P>0.05)。结论:IFNα-2b可以抑制大鼠子宫内膜异位病灶的血管生长,使异位内膜萎缩。更多还原

【Abstract】 Objective:To determine the effect of human interferon(IFN) -α-2b on endometriosis in rats and compare the two treatments.Methods:Thirty-eight rats with endometriosis were divided randomly into three groups which included group A(n=15) ,group B(n=15) ,and control group(n=8) .The rats in group A received 100 000 U of IFN-α-2b by intraperi-toneal(IP) deposit during the laparotomy.The rats in group B were treated with three doses of IFN(100 000 U every 48 hours) by SC injection after the second laparotomy was performed.The control group was not given any treatment.Laparotomy was performed to measure the size of each plant at week 4,6 and 8 after treatment.Using SP immunohistochemical technique,VEGF and MVD in ectopic endometrum of model rats were assessed.Results:At the last laparotomy,the mean size of the plants by animal were significantly smaller in the treatment groups than that in control group(P < 0.05) .The expression of VEGF and MVD in the ectopic endometrum of model rats were decreased in treatment groups than that in control group(P < 0.05) .There was no difference between two treatment groups in the mean volume,VEGF and MVD at the last laparotomy af-ter the treatment of IFN(P > 0.05) .Conclusion:Two short regimens of human IFN-α-2b reduce the size of experimental en-dometriosis in rats,perhaps by inhibiting the angiogenesis of ectopic endometrium.更多还原