【摘要】 目的研究p63、p73蛋白在宫颈癌及癌前病变中的表达及两者的相关性,初步探讨p63、p73基因在宫颈肿瘤发生、发展过程中的作用。方法采用免疫组化方法检测12例正常宫颈组织、38例宫颈上皮内瘤变(CIN)、32例宫颈鳞癌和8例宫颈腺癌患者的病理组织切片中p63、p73蛋白的表达情况,分析两者与相关临床病理参数间的关系及两者之间的相关性。结果p63蛋白定位于细胞核内,主要表达在正常宫颈鳞状上皮基底细胞和旁基底细胞、增生的储备细胞、不典型增生的鳞状上皮细胞和鳞癌组织中。在正常组、宫颈上皮内瘤变Ⅰ级组(CINⅠ)、宫颈上皮内瘤变Ⅱ级组(CINⅡ)、宫颈上皮内瘤变Ⅲ级组(CINⅢ)和鳞癌组中的阳性率分别为:8.3%、44.4%、40.0%、47.4%和68.8%,正常组、CIN组和鳞癌组之间差异有显著性意义。p73蛋白定位于细胞核内,主要表达在宫颈鳞状上皮的基底细胞中,在鳞癌癌巢中散在分布。在正常组、CINⅠ组、CINⅡ组、CINⅢ组和鳞癌组中的阳性率分别为:91.7%、88.9%、80.0%、73.3%和46.9%,正常组和CIN组与鳞癌组之间差异有显著性意义。在正常组、CIN组和鳞癌组之间p63、p73蛋白表达的阳性率呈负相关。在宫颈癌中,p63蛋白的阳性率与组织学类型、分级有关,与发病年龄、临床分期、淋巴结转移、浸润程度及肿瘤大小无关;p73蛋白的阳性率仅与组织学类型有关。结论p63高表达于鳞状细胞中,可作为宫颈鳞癌及具有向鳞状上皮分化倾向的增生储备细胞的标志物。宫颈鳞癌组织中p63明显高于正常宫颈组织,而p73明显低于正常宫颈组织,提示在宫颈癌的发生、发展过程中,两者可能起到了相互拮抗的作用。更多还原

【Abstract】 Objective To investigate the expression of p63 and p73 in carcinoma of cervix uteri and cervical intraepithelial neoplasia(CIN) and their relationship with clinicopathology.Methods Immunohistochemical techniques were used to detect the expression of p63 and p73 in 12 cases of normal cervix,38 cases of CIN,32 cases of squamous cell carcinoma of cervix uteri and 8 cases of adenocarcinoma.Results p63 was expressed in the basal,parabasal and reserve cells with high proliferative potentiality.The expression rate of p63 was 8.3% in normal cervix squamous cancer cells,44.4% in CINⅠ,40.0% in CINⅡ,47.4% in CINⅢ and 68.8% in cervical cancer respectively.p73 was expressed in the basal cells and squamous cancer cells.The expression rate of p73 protein was 91.7% in normal cervix squamous cancer cells,88.9% in CINⅠ,80.0% in CINⅡ,73.3% in CINⅢ and 56.2% in cervical cancer respectively.The expression rate of p63 was related to the pathological classification and histological grade,while p73 was only related to the pathological classification.The expression rate of p73 was inversely related to that of p63.Conclusion p63 may be a marker of squamous cell carcinoma of cervix uteri and proliferative reserve cells.p63 and p73 possibly have correlation with the pathogenesis and progress of squamous cervical cancer.更多还原