【摘要】 目的:从器官、组织的不同水平测定大鼠Ghrelin作用下胃肠动力的变化。方法:运用BD2000作为胃肠标记物,同时测定胃残留率和小肠推进比;多导生理仪记录大鼠胃窦肌电活动;多导生理仪记录离体大鼠胃体上1/3起搏区[带有ICC(interstitial cells of Caja)的胃肌]和胃窦环行肌肌条收缩波改变。结果:给药组大鼠胃残留量较对照组明显减少,给药组胃电慢波振幅及频率显著高于对照组,快波频率也增多。在组织水平,在Ghrelin作用下给药组离体的动物胃体起搏区及胃窦环行平滑肌肌条收缩幅度明显高于对照组;给药组胃体起搏区肌条的收缩频率与对照组无明显差别;给药组胃窦环行平滑肌条的收缩频率明显高于对照组,与其在体实验中胃排空明显增强,动力升高相吻合。结论:Ghrelin能增强胃肠动力,促进胃排空。更多还原

【Abstract】 Objective:To investigate the action of Ghrelin to gastrointestinal motility at organ and tissue.Methods:The gastric residual rate and intestinal propulsive ratio were measured using BD2000 as marker,at the same time gastromyoelectrical activity of the rat sinus ventriculi were recorded with Biopac MP150 systems.The contraction in the isolated gastric corpus’s(superior1/3 part with interstitial cells of Cajal)and antral circular muscle strips were recorded.Results:There were obvious decreases of the gastric residual rates and obvious increases of intestinal propulsive ratios in experimental group,compared with the control group.The frequencies and amplitudes of slow wave,frequencies of fast wave at the antrum ascended in experimental group and the differences of frequesncies and amplitudes were obvious compared with the control group.Under the action of Ghrelin,the degrees of contraction in the isolated gastric corpus’s(superior 1/3 part) and antral circular muscle strips were obviously higher than the control group.The frequency of contraction in the isolated gastric corpus’s(superior 1/3 part with interstitial cells of Cajal)circular muscle strips was no difference,but in antral circular muscle strips was higher than the control group compared with the control group.Conclusion:Ghrelin can reforce the gastrointestinal motility and promote gastric emptying and intestinal propulsion.更多还原