【摘要】 目的研究慢性肾衰竭(chronic renal failure,CRF)患者血浆总同型半胱氨酸(total homocysteine,tHcy)浓度的变化,并对其相关因素进行分析。方法采用荧光偏振免疫分析法检测122例CRF患者和130例正常对照组血浆tHcy浓度,同时检测其血清叶酸、VitB12浓度,并对它们之间的关系进行分析。结果血浆tHcy浓度CRF患者显著高于正常对照组(P<0.01),且慢性肾病(chronic kidney disease,CKD)Ⅴ期患者显著高于Ⅳ期患者(P<0.01),Ⅳ期患者显著高于Ⅲ期患者(P<0.01);正常对照组中血浆tHcy浓度男性显著高于女性(P<0.05),而CRF患者组血浆tHcy浓度男性略高于女性,但无显著性差异(P>0.05);血清叶酸浓度、VitB12浓度CRF患者均显著低于正常对照组(P<0.01)。相关分析显示,两组血浆tHcy浓度与血清叶酸、VitB12浓度均呈负相关性。结论CRF患者血浆tHcy浓度明显升高,且随着病情的加重,血浆tHcy浓度呈逐渐上升趋势,检测血浆tHcy浓度对了解CRF患者病情具有重要意义。叶酸、VitB12缺乏可能是诱发CRF患者血浆tHcy浓度升高的重要因素之一。更多还原

【Abstract】 Objective To study the concentration of plasma total homocysteine(tHcy)in patients with chronic renal failure(CRF)and discuss the correlative factors.Methods Plasma tHcy levels of 122 CRF patients and 130 normal control were determined using fluorescence polarization immunoassay(FPIA)and serum folic acid and VitB12 concentrations were measured.The relationships between them were also analyzed.Results The mean level of tHcy in CRF patients was significantly higher than that in normal controls(P<0.01),and the level of plasma tHcy was significantly higher in stage Ⅴ than in stage Ⅳ in chronic kidney disease(CKD)patients,and the level of plasma tHcy was significantly higher in stage Ⅳ than in stage Ⅲ in CKD patients.And in normal controls the plasma tHcy in male was significantly higher than that in female(P<0.05),but in CRF patients the plasma tHcy in male were slightly higher than that in female,the difference was not statistically significant(P>0.05).The mean level of serum folic acid and VitB12 in CRF patients were significantly lower than that in normal controls(P<0.01).Correlative analysis showed that plasma tHcy were negatively correlated with serum folic acid and VitB12.Conclusion The level of plasma tHcy were significantly elevated in patients with CRF,and there is a trend that the level of plasma tHcy increased with severity of CKD,and measuring the level of plasma tHcy has great value to know the degree of CKD.Deficiency of folic acid and VitB12 maybe important factors in inducing high homocysteinemia.更多还原