【摘要】 建立了多宝鱼和紫菜中砷甜菜碱的高效液相色谱多级质谱联用(HPLC-MS/MS)分析方法。样品以甲醇-水混合液(体积比1∶1)提取,提取液再经氮吹浓缩。样品溶液在C18柱上以1%(体积分数)甲酸和甲醇梯度洗脱,然后在电喷雾电离正离子模式下进行质谱分析。多反应监测模式下进行检测,标准曲线法定量。定量分析的线性范围为10~700μg/L,检出限为4.1μg/L,鱼和紫菜中的加标回收率分别为95%和68%。该文同时还研究了砷甜菜碱的碰撞裂解机理。更多还原

【Abstract】 A high performance liquid chromatography-tandem mass spectrometric method was developed for the determination of arsenobetaine in turbot(Scophthatmus maximus) and porphyra.The samples were extracted with 50 ∶50(by volume)water-methanol.The chromatographic separation was performed on a Phenomenex Luna C18(2)(4.6 × 250 mm,5 μm) column by gradient elution with 1%(by volume)formic acid and methanol.The eluent was then analyzed by the MSD TRAP XCT system(Agilent)with electrospray ionization positive ion mode.The collision-induced dissociation(CID) mechanism of arsenobetaine was discussed.The linear range of the calibration curve under MRM(multiple reaction monitoring) mode was 10-700 μg/L with a detection limit of 4.1 μg/L.Spiked recoveries for turbot and porphyra were 95% and 68%,respectively.更多还原