【摘要】 目的确定柴胡有效成分提取的最佳工艺。方法以柴胡中挥发油吸收度和柴胡皂苷a的含量为指标,采用单因素实验筛选主要因素和水平,再以正交实验筛选柴胡有效成分提取最佳工艺。结果最佳工艺参数为40℃温浸,收集相当于药材2倍量蒸馏液,继用8倍量含5%氨水的95%乙醇回流1 h提取柴胡皂苷,可使挥发油吸收度达0.941,柴胡皂苷a的含量达0.32%。结论该工艺为柴胡有效成分的提取提供了可靠依据,且工艺条件稳定可靠,重复性好。更多还原

【Abstract】 Objective To determine the optimum technology of active ingredients from Bupleurum chinense DC.Methods With the absorbance of volatile oils and the content of saikosaponin a in Bupleurum chinense DC as indexes,the major influencing factors and levels were observed by single factor experiment,and the optimum technology was optimized by orthogonal experiment.Results The optimum condition was as follows: the impregnating temperature was 40℃,the volume of distilled fluid was 2 times of weight of Bupleurum chinense DC,and the volume of 95%alcohol with 5%ammonia water to reflux extraction for 1hour was 8 times of weight of Bupleurum chinense DC,then the absorbance of volatile oils was 0.941 and the content of saikosaponin a was 0.32%.Conclusion The optimum technology provides reliable evidence for extraction of active ingredients from Bupuleurum chinense DC,and the conditions are accurate and reliable with good reproducibility.更多还原