【摘要】 目的研究大鼠灌胃给予当归补血汤后阿魏酸在体内的组织分布特性。方法采用高效液相色谱法;色谱柱为Kro-masil C18(4.6mm×150mm,5μm);流动相为0.2%甲醇乙腈-0.2%磷酸0.2%三乙胺水溶液(18:82);检测波长322nm;柱温40℃;流速0.8ml/min。结果各组织中的阿魏酸在10~400ng范围内与峰面积呈良好的线性关系;回收率平均为87.97%±1.95%;日内和日间的RSD分别1.92%~3.87%和3.21%~6.39%之间。阿魏酸在各组织器官中浓度差异较大。结论大鼠灌胃给予当归补血汤后阿魏酸主要在肝脏和肾脏分布,阿魏酸不能通过血脑屏障。更多还原

【Abstract】 Objective To investigate the tissue distribution of ferulic acid in rats after oral administration of Tangkuei blood-supplementing decoction.MethodsHPLC was used;Column: Kromasil C18(150mm×4.6mm,5μm);mobile phase: 0.2%methanol acetonitrile-0.2% phosphoric acid 0.2%triethylamine solution(18:82);detection wavelength was at 322nm;temperature was at 40℃;flow velocity was 0.8ml/min.ResultsThe linear ranges of ferulic acid in the heart, liver,spleen,lung and kidney were 10~400ng.The recovery of ferulic acid was 87.97%±1.95% on average.The intra-day and inter-day RSD were 1.92%~3.87% and 3.21%~6.39%,respectively.The concentrations of ferulic acid were very different in organs.ConclusionThe major distribution organs of ferulic acid in rats are liver and kidney.Ferulic acid can not pass though the blood-barrier.更多还原