【摘要】 目的对东亚钳蝎(Buthus martensii Karsch)神经毒素基因进行多序列比对,找到基因的结构特征,在此基础上对序列进行聚类分析,进而推测基因结构与功能的关系。方法搜集目前已知的东亚钳蝎神经毒素基因序列,利用Clustal X1.83软件进行多序列联配,然后用MEGA软件对基因组基因进行聚类分析。结果与结论东亚钳蝎神经毒素基因结构与功能密切相关,而且结构符合断裂基因的基本规律,如果这些基因结构基本一致,是由2个外显子和1个内含子组成,内含子的位置保守,位于信号肽中,基因长度的变化由内含子长度决定。更多还原

【Abstract】 Objective To carry out the multiple sequences alignment of the toxins’ gene from the Chinese scorpion Buthus martensii Karsch,and find out the gene structure.Methods The toxin genes were collected from the Chinese scorpion Buthus martensii Karsch,and the genetic distances and relatedness of the peptides were calculated using the program ClustalX 1.8.The neighbor joining tree was constructed by MEGA software.Results and conclusions The function of Buthus martensii Karsch toxins are related closely with their genes.And the genes are consistent with the basic rule of interrupted genes.更多还原