【摘要】 目的研究机体老化对树突状细胞(DCs)分化和功能的影响,为抗衰老、老年性疾病的治疗提供新思路。方法体外培养快速老化小鼠(SAMP1)骨髓来源的DCs,同时用来自同一基因型的正常小鼠(C3H/HeJ)做对照,研究SAMP1小鼠的DCs分化能力及成熟DCs促淋巴细胞增殖的能力。结果流式细胞仪分析显示,SAMP1小鼠和C3H/HeJ小鼠骨髓来源的成熟DCs表达CD80和MHCⅡ类分子的能力无统计学差异。淋巴细胞的增殖实验结果提示伴刀豆球蛋白A(ConA)组和核苷酸(CPG)组差异无显著性,仅内毒素(LPS)组有显著差异,且老化小鼠具有更强的刺激能力。结论老化小鼠骨髓来源的DCs的分化及功能并无明显下降。更多还原

【Abstract】 Objective To evaluate the differentiation and function of the bone marrow-derived dendritic cells(BM-DCs) of senescence-accelerated mouse prone(SAMP1). Methods Bone marrow cells of SAMP1 and C3H/HeJ mice which had the same MHC background with SAMP1,were cultured in the presence of recombinant mouse granulocyte-macrophage colony-stimulating factor(rmGM-CSF) and interleukin-4(IL-4) for 6 d.Lipopolysaccharide(LPS) was added and cultured for additional 2 d to induce the matured DCs.The expressions of CD80 and MHC class Ⅱ were examined by flow cytometry.The capacity of proliferation of allogeneic T cells stimulated by BM-DCs was evaluated by mixed lymphocytes reaction(MLR). Results Both marrow cells of SAMP1 and C3H/HeJ mice had capability in differentiating into DCs in vitro in the presence of rmGM-CSF and IL-4.The expressions of CD80 and MHC class Ⅱ of SAMP1 were comparable with that of C3H/HeJ mice.The result of MLR showed no significant difference in the capacity of proliferation of allogeneic T cells.Also,the splenocytes of SAMP1 and C3H/HeJ mice had the same capacity of proliferation stimulated by LPS,ConA and CpG-ODN. Conclusions These results suggest that there are no significant changes of differentiation and function of the BM-DCs of SAMP1.更多还原