【摘要】 目的探讨外源性肝细胞生长因子(HGF)能否调控肝癌细胞HEPG2 p16基因的表达水平。方法以人肝癌细胞株HepG2为研究对象,根据培养基中HGF的浓度不同,实验分组为:对照组、实验1组和实验2组,采用流式细胞仪(FCM)检测HGF的肝癌细胞生长周期;采用逆转录聚合酶链反应(RT-PCR)技术检测HGF对肝癌细胞p16基因表达的影响。结果HepG2细胞在10μg/L和50μg/L HGF的作用下,细胞周期出现明显改变,其中G0/G1期细胞明显多于对照组,而S期细胞减少。经HGF作用后肝癌细胞的p16基因表达上调。结论HGF通过上调p16表达阻滞细胞分裂于G0/G1期,可能为其抑制人肝癌细胞HepG2的生长作用的重要机制之一。更多还原

【Abstract】 Objective To study the effect of hepatocyte growth factor(HGF) on the expression level of gene P16 in HepG2 in vitro.Methods According to the different concentration of HGF,the cell line HepG2 were divided into 3 groups: control group,experiment group 1 and experiment group 2.The flow cytometry(FCM) was used to detect phase distribution of the cycles and RT-PCR technology for the expression of p16 gene.Results The cell cycles were changed obviously and especially S phase cells were significantly decreased as well as the quiescent G0/G1 phase cells were accumulated by raising HGF concentration.Percentages of S phase cells in 10mg/L and 50mg/L groups were(7.5 ± 4.4) %,(7.8 ±1.6) %,which were significantly lower than that of control group(16.6 ±2.8)%;the percentages of G0/G1 phase cells were(80.5±3.2)% and(73.1 ±3.9)%,which were significantly higher than that in control group(59.6 ±6.2)%.The levels of P16 gene were up-regulated in HGF group vs.control group.Conclusion HepG2 cell division is inhibited at G0/G1 phase by HGF,which maybe one of important mechanism of HGF inhibition of the proliferation and induction of the apoptosis in HepG2.更多还原