【摘要】 目的:观察亚低温对大鼠脑缺血后血管生成及血管内皮生长因子(VEGF)表达的影响,探讨亚低温治疗缺血性脑病的可行性。方法:66只大鼠随机分为假手术组、常温组和亚低温组。栓线法造模,亚低温组大鼠行低温(33～34℃)干预24h后自然复温。免疫组化染色检测缺血边缘区CD31、Ki67阳性细胞密度以及VEGF表达。结果:两组大鼠在脑缺血后各时间点CD31阳性细胞细胞计数无统计学差异;亚低温组Ki67阳性细胞密度与VEGF表达在低温干预期间低于常温组,复温之后两组无显著性差异。结论:亚低温抑制VEGF表达,脑缺血后短期的亚低温治疗不影响血管生成,推测VEGF主要在脑缺血24h后发挥促血管生成作用。更多还原

【Abstract】 Objective:To observe the effects of mild hypothermia on the expression of VEGF and angiogenesis in rats with cerebral ischemic disease.Methods:66 rats were divided randomly into 3 groups including sham-operated group,normothermia group and mild hypothermia group.Using nylon to establish cerebral ischemia model of rats,and rats in mild hypothermia group got the treatment of hypothermia(33~34℃)immediately as soon as the vessel was blocked.Then the density of CD31 positive-stain cells,the density of Ki67 positive-stain cells and the expression of VEGF protein were measured by immunohistochemical staining in each time point.Results:There was little difference in the density of CD31 positive-stain cells between the two groups in each time point.The density of Ki67 positive-stain cells and the expression of VEGF protein in mild hypothermia group were lower than that in normthermia group during the period of hypothermia,while the difference between the two groups was not obvious after rewarming.Conclusion:Mild hypothermia inhibits the expression of VEGF,and short-term mild hypothermia in early stage of cerebral ischemia has no effect on angiogenesis.It is presumed that VEGF promotes angiogenesis in 24 hours later after cerebral ischemia.更多还原