【摘要】 目的:通过对系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMC)中NK T细胞含量及其功能状态的研究,评价NK T细胞在这类疾病失调中的可能作用及其机制。方法:流式细胞光度术(FCM)检测NK T细胞含量;细胞培养检测NK T细胞增殖能力;ELISA检测细胞因子。结果:PBMC中NK T细胞含量比较:SLE组较正常对照组明显减少,具有极显著性差异。培养前后PBMC中NK T细胞含量比较,培养后较培养前均增加,具有极显著性差异。培养上清中IFN-γ含量比较:SLE组与正常对照组间比较无显著性差异。培养上清中IL-4含量比较:SLE组较正常对照组明显增加,具有极显著性差异。结论:SLE的发病可能与NK T细胞数量的低下及功能严重失调有关。更多还原

【Abstract】 Objects:To explore the changes of amount and function of Vα24-Vβ11 NK T cells in the patients with Systemic Lupus Erythematosus.Methods:The amount and proliferation of cultured NK T cells were tested by FCM;IFN-γ and IL-4 were tested by ELISA.Results:The amount of Vα24-Vβ11 NK T cells in the patients with SLE was much lower than that in controls;The levels of IFN-γ on SLE groups were as many as that in controls;The levels of IL-4 on SLE groups were higher than that in control.Conclusion :Pathognesis of SLE may be related to the amount of NK T cells and their serious maladjustment.更多还原